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Protein Folding and Misfolding

Despite the experimental and theoretical efforts of many laboratories in the last 40 years, our understanding of the basic rules that govern the molecular processes leading to folded and misfolded conformations of proteins remains highly limited. The advent of single-molecule techniques, such as optical tweezers, has provided an innovative perspective on the protein folding problem, allowing us to go beyond the ensemble average and dissect folding and misfolding mechanisms in unprecedented detail. In our laboratories we use optical tweezers to elucidate folding and misfolding pathways of individual proteins, and characterize the energy landscapes underlying these reactions. We are studying different proteins, including Acyl-CoA-binding protein, neuronal calcium sensor-1 and HIV-1-protease.

Overlay of refolding traces leading to the native state (red), and to two different misfolded conformations (M1 and M2) of the neuronal calcium sensor-1.)

[Ultimo aggiornamento: 01/07/2015 18:59:56]